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S9305

Sigma-Aldrich

SYBR® Green II RNA gel stain

greener alternative

10,000 × in DMSO

Synonyme(s) :

RNA gel dye, SYBR® RNA dye, safer gel stain

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About This Item

Numéro CAS:
172827-25-7
Numéro CE :
200-664-3
Numéro MDL:
MFCD00284716
Code UNSPSC :
12171500
Nomenclature NACRES :
NA.52

Utilisation

1 mL sufficient for 100 mini-gels

Niveau de qualité

100

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Concentration

10,000 × in DMSO

Autre catégorie plus écologique

Aligned,

Température de stockage

−20°C

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Description générale

SYBR® Green II is a highly sensitive stain for post electrophoresis staining of RNA and ssDNA in agarose or polyacrylamide gels. SYBR® Green II is not selective for RNA staining but does exhibit a higher quantum yield when bound to RNA than to double stranded DNA.
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has inherently safer chemistry, compared to the standard use of ethidium bromide for staining. For more information see publication found in related content.

Application

SYBR® Green II RNA gel stain has been used:

  • for the quantification of ribonucleic acid (RNA) in Escherichia coli
  • for staining 5′-ETS rRNA species separated on polyacrylamide gel prior to Northern blot analysis
  • to stain formaldehyde agarose gels to visualize RNA bands

Caractéristiques et avantages

  • Ultrasensitive stain for post-electrophoresis staining of RNA and ssDNA
  • Excited maximally at 497 nm with a secondary excitation peak at 254nm
  • The fluorescence emission of SYBR®Green II stained RNA is centered at 520 nm
  • Staining agarose/formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or subsequent hybridization in Northern blot analysis as long as 0.1% -0.3% SDS is included in prehybridization and hybridization buffers to remove the dye
  • Might facilitate the detection of viroid RNAs and multicopy cellular RNA species
  • More sensitive than ethidium bromide in applications that require extremely sensitive detection techniques such as single-strand conformation polymorphism (SSCP) analysis

Informations légales

SYBR is a registered trademark of Life Technologies

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Réf. du produit
Description
Tarif

Classe de danger pour l'eau (WGK)

WGK 3

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
MKCV5338
MKCT3288
MKCS1336
MKCP9666
MKCL4011

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Consulter la Bibliothèque de documents

Benjamin Lau et al.
Molecular cell, 81(2), 293-303 (2020-12-17)
Ribosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in this progression is removal of the 5' external transcribed spacer (5'-ETS), an RNA extension
Human ??defensin-1 and -2 expression in the gingiva of patients with specific periodontal diseases
S. Vardar-Sengul
Journal of Periodontal Research (2007)
Michael Richmond et al.
Biochemistry, 50(12), 2298-2312 (2011-02-09)
In this work, we describe RapA-dependent polyadenylation of model RNA substrates and endogenous, RNA polymerase-associated nucleic acid fragments. We demonstrate that the Escherichia coli RNA polymerase obtained through the classic purification procedure carries endogenous RNA oligonucleotides, which, in the presence
Dyes for in vitro detection of nucleic acids and proteins.
BioProbes, 20, 12-13 (1994)
L G Lee et al.
Cytometry, 7(6), 508-517 (1986-11-01)
The purpose of this study was to find a 488-nm excitable fluorescent dye for reticulocyte analysis by the use of fluorescence activated cell cytometry. The chemical structure of thioflavin T, a dye used for reticulocyte analysis with 457-nm excitation, was
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