A5912

N,N′-Bis(acryloyl)cystamine

~98% (HPLC)

• Synonyme(s) : 2,2′-(Bisacrylamino)diethyl disulfide, BAC, Bis(2-acrylamidoethyl) disulfide
• Formule empirique (notation de Hill) : C₁₀H₁₆N₂O₂S₂
• Numéro CAS: 60984-57-8
• Poids moléculaire : 260.38
• Numéro CE : 262-546-8
• Numéro MDL: MFCD00036225
• Code UNSPSC : 12352200

Propriétés

• Essai: ~98% (HPLC)
• Solubilité: acetic acid: 50 mg/mL
• Température de stockage: −20°C
• Chaîne SMILES: C=CC(=O)NCCSSCCNC(=O)C=C
• InChI: 1S/C10H16N2O2S2/c1-3-9(13)11-5-7-15-16-8-6-12-10(14)4-2/h3-4H,1-2,5-8H2,(H,11,13)(H,12,14)
• Clé InChI: DJVKJGIZQFBFGS-UHFFFAOYSA-N

Description

Application

BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.

BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves