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A2304

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Goat Anti-Mouse IgG (Fab specific)- HRP

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About This Item

Numéro MDL:
MFCD00162623
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Conjugué

peroxidase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

mouse

Ne doit pas réagir avec

bovine, horse, human

Technique(s)

direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue section
western blot: 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Catégories apparentées

Description générale

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense and is a glycoprotein. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody associates with mouse IgG and mouse Fab fragment. The conjugate shows no reactivity with mouse Fc fragment, human IgG, IgA, IgM, kappa and lambda light chain, bovine IgG and IgM, or horse IgG.

Application

Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.
Anti-Mouse IgG (Fab specific)–Peroxidase antibody has been used in western blotting.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody is suitable for use in immunohistochemistry applications at 1:200 dilution using human tonsil tissues. The antibody can also be used for western blot assays at 1:80,000-1:160,000 dilutions using total cell extract of HeLa cells.

Autres remarques

Antibody adsorbed with bovine, equine, and human serum proteins.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Notes préparatoires

Adsorbed to reduce background staining with bovine, horse and human samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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