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Sigma-Aldrich

EMPIGEN® BB detergent

~30% active substance

Synonyme(s) :

N,N-Dimethyl-N-dodecylglycine betaine, N-(Alkyl C10-C16)-N,N-dimethylglycine betaine

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About This Item

Formule linéaire :
CH3(CH2)8-14CH2N+(CH3)2CH2COO-
Numéro CAS:
66455-29-6
Numéro Beilstein :
3670807
Numéro CE :
266-368-1
Numéro MDL:
MFCD00084742
Code UNSPSC :
12161900
ID de substance PubChem :
329753464
Nomenclature NACRES :
NA.25

Description

zwitterionic

Niveau de qualité

200

Forme

liquid

Composition

solid content, 35.0-45.0 % (w/v)

Concentration

~30% active substance

CMC

1.6-2.1 mM (20-25°C)

Densité

1.030-1.060 g/mL at 20 °C

Absorption UV

λ: 417 nm Amax: ≤0.055
λ: 441 nm Amax: ≤0.037

Chaîne SMILES 

CCCCCCCCCCCC[N+](C)(C)CC([O-])=O

InChI

1S/C16H33NO2/c1-4-5-6-7-8-9-10-11-12-13-14-17(2,3)15-16(18)19/h4-15H2,1-3H3

Clé InChI

DVEKCXOJTLDBFE-UHFFFAOYSA-N

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Catégories apparentées

Application

Empigen BB is a zwitterionic detergent compatible with anionic, non-ionic, cationic and other amphoteric surfactants.

Empigen BB is a useful detergent that has been used in the solubilization of membrane proteins and biochemical analysis of keratins. It not only helps to solubilize bigger amounts of protein but it also maintains antibody reactivity. It has been also used in the purification of leucine-rich repeat kinase 2 (LRRK2) in Escherichia coli during research in Parkinson′s disease. The use of Empigen in the preparation of viral antigens is being increasing due to its effectivity in giving greater antibody responses.

Informations légales

EMPIGEN is a registered trademark of Huntsman Corporation

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319,H335

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

James K Wahl
Hybridoma and hybridomics, 21(1), 37-44 (2002-05-07)
Desmosomes are the most prominent cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Desmogleins and desmocollins are the transmembrane core of the desmosome and both are members of the cadherin family
Wenjin Guo et al.
Protein expression and purification, 90(1), 34-39 (2013-05-15)
Vaccinia virus (VV) has been used to express a variety of heterologous proteins, including HIV envelope (Env) glycoproteins. The Env protein is synthesized as a precursor molecule, gp160, which is cleaved into the surface antigen gp120 and the transmembrane protein
Y Rinke et al.
Virology, 170(2), 424-432 (1989-06-01)
We have established a sensitive assay for the quantitative determination of large T antigen determinants on the surface of living simian virus 40 (SV40)-transformed cells (mKSA). Cells in suspension culture were incubated with monoclonal antibodies specific for large T antigen
M Lukácová et al.
European journal of epidemiology, 10(2), 227-230 (1994-04-01)
A 29 kDa protein, isolated from the outer membrane of Coxiella burnetii, strain Nine Mile phase I by detergent Empigen BB, was characterized. The failure in removing lipopolysaccharides (LPS) from preparations of the protein by the purification method used indicates
Adam M Rotunda et al.
Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.], 30(7), 1001-1008 (2004-06-24)
Phosphatidylcholine injections are becoming an increasingly popular technique to treat localized fat accumulation. This formula is composed primarily of phosphatidylcholine and sodium deoxycholate, a bile salt used to solubilize the natural phospholipid in water. The mechanism through which this injectable
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