M0303
Middlebrook 7H10 Agar Base
suitable for microbiology, NutriSelect® Plus, for use in isolation and cultivation of Mycobacterium species
Synonyme(s) :
Mycobacterial culture media
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About This Item
Code UNSPSC :
41171609
Nomenclature NACRES :
NA.85
Produits recommandés
Niveau de qualité
Stérilité
non-sterile
Gamme de produits
BioChemika
Forme
powder
Durée de conservation
limited shelf life, expiry date on the label
Conditionnement
pkg of 500 g
Fabricant/nom de marque
NutriSelect® Plus
Technique(s)
microbe id | susceptibility testing: suitable
microbiological culture: suitable
pH final
6.6±0.2 (25 °C)
Application(s)
clinical testing
food and beverages
life science and biopharma
veterinary
microbiology
Adéquation
mycobacteria
Catégories apparentées
Description générale
Middlebrook 7H10 Agar Base is recommended for isolation, cultivation and sensitivity testing of Mycobacterium tuberculosis. Middlebrook media consists of many inorganic salts, which help, in growth of Mycobacteria. Citric acid formed from sodium citrate helps in retaining inorganic cations in solution. Glycerol supplies carbon and energy. Middlebrook OADC Growth Supplement (M0678) contains oleic acid, bovine albumin, sodium chloride, dextrose and catalase. Oleic acid and other long chain fatty acids are essential for metabolism of Mycobacteria. Some free fatty acids are toxic to Mycobacteria, but albumin binds to those fatty acids and prevents toxic action on Mycobacteria. Dextrose serves as an energy source. Catalase neutralizes toxic peroxides. Malachite green partially inhibits other bacteria.
Application
Middlebrook 7H10 Agar Base is a selective media for isolation, cultivation and sensitivity testing of Mycobacterium tuberculosis. Mycobacteria grow most rapidly in broth media and hence primary isolation of bacterial species can be carried out. It is inexpensive, easy to prepare and can aid in early detection of acid-fast, Mycobacterium species by culture.
Composants
Ingredients (g/L)
Ammonium sulfate, 0.50
L-Glutamic acid, 0.50
Monopotassium phosphate, 1.50
Disodium phosphate, 1.50
Sodium citrate, 0.40
Ferric ammonium citrate, 0.04
Magnesium sulfate, 0.025
Calcium chloride, 0.0005
Zinc sulfate, 0.001
Copper sulfate, 0.001
Pyridoxine hydrochloride, 0.001
Biotin, 0.0005
Malachite green, 0.00025
Agar, 15.00
Ammonium sulfate, 0.50
L-Glutamic acid, 0.50
Monopotassium phosphate, 1.50
Disodium phosphate, 1.50
Sodium citrate, 0.40
Ferric ammonium citrate, 0.04
Magnesium sulfate, 0.025
Calcium chloride, 0.0005
Zinc sulfate, 0.001
Copper sulfate, 0.001
Pyridoxine hydrochloride, 0.001
Biotin, 0.0005
Malachite green, 0.00025
Agar, 15.00
Notes préparatoires
Suspend 19.47 g of Middlebrook 7H10 Agar Base in 900 ml of distilled water. Add 5 ml of glycerol (Cat. No. 49767). Boil to dissolve the medium completely. Distribute in 180 ml amounts in erlenmeyer flasks and sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes. Cool to 45°C and aseptically add 20 ml of Middlebrook OADC Growth Supplement (Cat. No. M0678). Mix well and pour into screw capped tubes.Note: Keep prepared medium in the dark before and after inoculation.
Note de bas de page
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Informations légales
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Produit(s) apparenté(s)
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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