11467140001
Roche
DNA, MB-grade
from fish sperm
Synonyme(s) :
DNA
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About This Item
Code UNSPSC :
41106310
Produits recommandés
Source biologique
fish (sperm)
Niveau de qualité
Qualité
Molecular Biology
Forme
solution (ready-to-use)
Poids mol.
40-1,000 kDa
Conditionnement
pkg of 500 mg
Fabricant/nom de marque
Roche
Concentration
100 μg/mL
Couleur
light yellowopalescent
Solubilité
water: miscible
Température de stockage
−20°C
Description générale
DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.
DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture.
Application
The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.
Séquence
Chain Length 120 to 3,000 nucleotides
Forme physique
Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0
Notes préparatoires
Working concentration: 100μg/ml
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
No data available
Point d'éclair (°C)
No data available
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