MABT121

Anti-Vimentin Antibody, clone 3CB2

clone 3CB2, from mouse

• Synonyme(s) : Vimentin
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 3CB2, monoclonal
• Espèces réactives: chameleon (GLIA 15:389-400 (1995), Prada, F. A., et. al.), rat (GLIA 15:389-400 (1995), Prada, F. A., et. al.), human, lizard
• Réactivité de l'espèce (prédite par homologie): chicken (immunogen homology)
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgMκ
• Numéro d'accès NCBI: NP_001041541
• Numéro d'accès UniProt: P09654
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... VIM(7431)

Description

Description générale

Vimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments.

Immunogène

Vimentin extracted from chicken embryonic brain tissue.

Application

Anti-Vimentin Antibody, clone 3CB2 is an antibody against Vimentin for use in WB, IC, IH.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in human central and peripheral retinal sections (Perez-Alvarez, M.J., et al. (2008). J Neurosci Res. 86(8):1871-1883).

Western Blot Analysis:
A representative lot was used to detect vimentin in chicken and rat total brain extracts (Prada, FA., et al. (1995). GLIA 15:389-400).

Immunohistochemistry Analysis:
A representative lot was used to detect vimentin in E3 chick embryo sections and different regions of the developing chick central nervous system (Prada, FA., et al. (1995). GLIA 15:389-400).

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Qualité

Evaluated by Western Blot in ARPE19 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Vimentin in ARPE19 cell lysate.

Description de la cible

~54 kDa observed

Forme physique

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
ARPE cell lysate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable