MABS1241

Anti-Synembryn-A/Ric8A Antibody, clone 3E1

clone 3E1, from mouse

• Synonyme(s) : Protein Ric-8A, Resistance to inhibitors of cholinesterase 8
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 3E1, monoclonal
• Espèces réactives: rat, human
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès UniProt: Q80ZG1
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RIC8A(60626)

Description

Description générale

Synembryn-A (UniProt: Q80ZG1; also known as Resistance to inhibitors of cholinesterase 8, Protein Ric-8A) is encoded by the Ric8a gene (Gene ID: 293614) in rat species. Resistance to inhibitors of cholinesterase 8 (RIC-8) is conserved in vertebrates and is concentrated in the cytoplasm of neurons. Mammalian Ric-8 proteins are G alpha subunit guanine nucleotide exchange factors. Ric-8A and Ric-8B collectively stimulate nucleotide exchange of all G alpha subunit classes by stabilizing the G alpha nucleotide-free transition state. Ric-8 is reported to directly participate in the post-translational folding of nascent G alpha subunits to promote G-protein biosynthesis. It may facilitate the transition of G alpha from a pre-folded globular state to its native state by promoting the first G alpha guanine nucleotide-binding event. Ric-8 interacts with GDP-bound G alpha proteins, but does not interact with G-alpha proteins when they are in complex with beta and gamma subunits. Overexpression of Ric-8 proteins in HEK293, NIH 3T3, or Sf9 cells is shown to enhance cellular G alpha levels. Embryonic stem cells lacking Ric-8A or Ric-8B produce defective G alpha subunits that turn over at about 10-fold higher rates than in wild-type cells. Ref.: Thomas, C.J. et al. (2011). PLoS ONE 6(8): e23197.

Spécificité

Clone 3E1 detects Ric-8A in human brain.

Immunogène

A full-length recombinant Ric-8A protein from rat.

Application

Analyse par immunohistochimie :

A representative lot detected recombinant Synembryn-A/Ric-8A protein (Chan, P.Y., et al. (2013). Proc. Natl. Acad. Sci. USA. 110(10):3794-3799).

Detect Synembryn-A/Ric-8A using this mouse monoclonal Anti-Synembryn-A/Ric8A antibody, clone 3E1, Cat. No. MABS1241, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Qualité

Produit évalué par western blotting sur du lysat de tissu cérébral humain.

Analyse par western blotting : µµ

Description de la cible

Poids réel (observé) : env. 60 kDa ; poids théorique (calculé) : 59,7 kDa. Des bandes non caractérisées peuvent être observées avec certains lysats.

Forme physique

Format : Produit purifié

Autres remarques

Concentration : Voir la fiche technique du lot concerné.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1