MABE977

Anti-E3 ubiquitin-protein ligase UHRF1, clone BF18.11 Antibody

clone BF18.11, from mouse

• Synonyme(s) : E3 ubiquitin-protein ligase UHRF1, Nuclear protein 95, Nuclear zinc finger protein Np95, Ubiquitin-like PHD and RING finger domain-containing protein 1, mUhrf1, Ubiquitin-like-containing PHD and RING finger domains protein 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: BF18.11, monoclonal
• Espèces réactives: mouse
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001104548
• Numéro d'accès UniProt: Q8VDF2
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Uhrf1(18140)

Description

Description générale

Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.

Immunogène

His-tagged recombinant protein corresponding to mouse E3 ubiquitin-protein ligase UHRF1.

Application

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected E3 ubiquitin-protein ligase UHRF1 in NIH/3T3 cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated E3 ubiquitin-protein ligase UHRF1 in 5 mg of NIH/3T3 cell lysate (Roberto Papait, FIRC Institute of Molecolar Oncology).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-E3 ubiquitin-protein ligase UHRF1, clone BF18.11 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunoprecipitation for the detection of E3 ubiquitin-protein ligase UHRF1.

Qualité

Evaluated by Western Blotting in NIH-3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected E3 ubiquitin-protein ligase UHRF1 in 10 µg of NIH-3T3 cell lysate.

Description de la cible

~88 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable