CP28
Anti-Fibroblast Antigen Mouse mAb (AS02)
liquid, clone AS02, Calbiochem®
Synonyme(s) :
Anti-CD90 (Ab-1) (Mouse), Anti-Thy-1 (Ab-1) (Mouse)
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About This Item
Code UNSPSC :
12352203
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
AS02, monoclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
human, monkey
Ne doit pas réagir avec
rat, mouse
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
Isotype
IgG1
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... FGF22(27006)
Description générale
Anti-Fibroblast Antigen, mouse monoclonal, clone AS02, detects ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts (non-reducing conditions). It is validated for FC, WB, IF, IP & IHC (frozen sections).
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with X63-Ag.8.653 mouse myeloma cells. Recognizes the ~30-31 kDa fibroblast antigen (CD90/Thy-1) under non-reducing conditions.
Recognizes the ~30-31 kDa CD90/Thy-1 antigen in human fibroblasts under non-reducing conditions.
Immunogène
Human
human fibroblasts
Application
Flow Cytometry (see comments)
Frozen Sections (10 µg/ml, acetone fixation)
Immunoblotting (10 µg/ml, non-reducing only)
Immunofluorescence (10 µg/ml)
Immunoprecipitation (2-10 µg/ml)
Frozen Sections (10 µg/ml, acetone fixation)
Immunoblotting (10 µg/ml, non-reducing only)
Immunofluorescence (10 µg/ml)
Immunoprecipitation (2-10 µg/ml)
Conditionnement
Please refer to vial label for lot-specific concentration.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In 50 mM sodium phosphate buffer, 6% BSA.
Remarque sur l'analyse
Positive Control
Any fibroblast cell line or skin tissue
Any fibroblast cell line or skin tissue
Autres remarques
Saalbach, A., et al. 1996 J. Invest. Dermatol.106, 1314.
Janin, A., et al. 1990. Clin. Exp. Rheum.8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol.92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol.38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem141, 477.
Janin, A., et al. 1990. Clin. Exp. Rheum.8, 237.
Kay, M., et al. 1989. J. Invest. Dermatol.92, 168.
Esterre, P.H., et al. 1984 Cell Mol. Biol.38, 297.
Hoyhtya, M., et al. 1984 Eur. J. Biochem141, 477.
This antibody has been used with magnetic beads to remove fibroblasts from mixed cultures. It is also suitable for flow cytometry studies (see application references). Note that in healthy tissue (skin, placenta, liver, heart), this antibody does not react with endothelial cells. However, endothelial cells activated by PMA, TNFα, or IFNγ may be detected by this antibody, suggesting activated endothelial cells may express CD90 and that CD90 may be an activation marker of human endothelial cells. Antibody should be titrated for optimal results in individual systems.
Informations légales
Manufactured by Dianova, GmbH, Germany.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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