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05-386

Sigma-Aldrich

Anti-Vinculin Antibody, clone V284

clone V284, Upstate®, from mouse

Synonyme(s) :

Anti-CMD1W, Anti-CMH15, Anti-HEL114, Anti-MV, Anti-MVCL

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

V284, monoclonal

Espèces réactives

human, rat, mouse, rabbit, chicken

Fabricant/nom de marque

Upstate®

Technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès NCBI

NM_003373

Numéro d'accès UniProt

P18206

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... VCL(7414)

Spécificité

Vinculin

Immunogène

Human Vinculin

Application

Anti-Vinculin Antibody, clone V284 detects level of Vinculin & has been published & validated for use in IP, WB, IH.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Qualité

routinely evaluated by immunoblot on RIPA lysates of human A431 cells, lysates from chicken embryonic fibroblasts (CEF), or mouse 3T3/A31 fibroblasts

Description de la cible

117kDa

Forme physique

Format: Purified
PBS, pH 7.4, containing 0.05% sodium azide and 1mg/ml BSA
Protein A chromatography

Stockage et stabilité

2 years at -20°C

Remarque sur l'analyse

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Paul G Leonard et al.
Nature chemical biology, 12(12), 1053-1058 (2016-10-25)
Despite being crucial for energy generation in most forms of life, few if any microbial antibiotics specifically inhibit glycolysis. To develop a specific inhibitor of the glycolytic enzyme enolase 2 (ENO2) for the treatment of cancers with deletion of ENO1
Unique morphology and focal adhesion development of valvular endothelial cells in static and fluid flow environments.
Butcher, JT; Penrod, AM; Garcia, AJ; Nerem, RM
Arteriosclerosis, Thrombosis, and Vascular Biology null
X Wu et al.
The Journal of biological chemistry, 276(32), 30285-30292 (2001-05-31)
The L-type calcium channel is the major calcium influx pathway in vascular smooth muscle and is regulated by integrin ligands, suggesting an important link between extracellular matrix and vascular tone regulation in tissue injury and remodeling. We examined the role
Zeinab Chitforoushzadeh et al.
Science signaling, 9(431), ra59-ra59 (2016-06-09)
Signal transduction networks coordinate transcriptional programs activated by diverse extracellular stimuli, such as growth factors and cytokines. Cells receive multiple stimuli simultaneously, and mapping how activation of the integrated signaling network affects gene expression is a challenge. We stimulated colon
Temperature dependent specific heat capacity (Cp) of G-actin and talin or talin-vinculin bound to G-actin.
W H Goldmann et al.
Biochemical Society transactions, 20(3), 273S-273S (1992-08-01)
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