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WHAWB120355

QIAcard Non-Indicating FTA Cards

mini, 2 sample areas per card, pkg of 25 cards

Synonyme(s) :

QIAcard FTA Mini (25), Whatman FTA, Z747521

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About This Item

Code UNSPSC :
41105500
Nomenclature NACRES :
NB.22

Description

FTA Mini Card with 2 sample areas per card, 125 μL maximum volume/sample area, 250 μL maximum total volume/card

Fabricant/nom de marque

Qiagen WB120355

Conditionnement

pkg of 25 cards

Adéquation

suitable for (isolation, purification and storage of nucleic acids)

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Description générale

FTA Cards offer a compact room-temperature storage system that reduces the need for precious freezer space.

QIAcard FTA Mini format with 2 spot area to collect, stabilize, process, transport, and archive colored samples such as blood. FTA technology enables cell lysis on contact, denatures proteins and immediate stabilization and protection of nucleic acids.

FTA mini cards utilize QIAcard FTA technology that simplifies the handling and processing of nucleic acids. FTA Cards contain chemicals that lyse cells, denature proteins and protect nucleic acids from nucleases, oxidation and UV damage. FTA cards rapidly inactivate organisms, including blood-borne pathogens, and prevent the growth of bacteria and other microorganisms.

Convenient for protocols that require different locations for testing and archiving samples. Different samples can be processed independently.

Application

Use FTA card technology for a wide range of applications:
  • Transgenic identification
  • Transfusion medicine
  • Plasmid screening
  • Food and agriculture testing
  • Drug discovery
  • Genomics
  • STR analysis
  • Animal identification
  • Whole genome amplification
  • Molecular biology

Caractéristiques et avantages

  • Two sample areas for application of up to 250 μl sample volume per card.
  • Simply apply your sample to the FTA Card. Cell membranes and organelles are lysed and the released nucleic acids are entrapped in the fibers of the matrix. The nucleic acids remain immobilized and are preserved for transport, immediate processing or long-term room temperature storage.
  • Since captured nucleic acids are preserved, FTA Cards facilitate sample collection in remote locations and simplify sample transport.
  • Ship your samples back to the laboratory without expensive special handling or dry ice and process at your convenience.
  • FTA Cards can be used with virtually any sample type: Blood, Cultured cells, Buccal cells, Plasmids, and Solid tissue.
  • Captured nucleic acids are ready for purification when you are. Just take a punch from the FTA Card, wash with FTA Purification. Reagent and rinse with TE-1 (10 mM Tris-HCI, 0.1 mM EDTA, pH 8) buffer. DNA on the washed punch is ready to use in applications such as PCR, SNP analysis, and real-time PCR. Since PCR products remain in solution, the punch can be used for multiple amplifications.

Autres remarques

Sample integrity is optimized when FTA Cards are stored in a Multi-Barrier Pouch with a Desiccant Packet.
Field of Use : For internal research use only

Informations légales

FTA is a trademark of Qiagen Group
QIAcard is a trademark of Qiagen Group

Certificats d'analyse (COA)

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Articles

Description of common problems that can occur during genomic DNA preparation

This article describes the evaluation of Whatman FTA cards from Cytiva for their ability to collect, store, and isolate high-quality RNA from a variety of crude biological samples.

Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

Protocoles

This study examined the yield and quality of DNA from samples applied to Whatman FTA cards, using five common methods of DNA extraction.

A number of options are available for sample collection, transport, archiving, and DNA purification.

Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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