Pular para o conteúdo
Merck
  • Facile preparation of polyvinyl alcohol coated SiO2 stationary phases for high performance liquid chromatography.

Facile preparation of polyvinyl alcohol coated SiO2 stationary phases for high performance liquid chromatography.

The Analyst (2014-09-03)
Shunli Ji, Feifang Zhang, Shengjie Wu, Bingcheng Yang, Xinmiao Liang
RESUMO

A facile method to prepare a polar stationary phase for hydrophilic interaction chromatography (HILIC) was proposed by coating polyvinyl alcohol onto silica particles (PVA-Sil). A water or organic solvent-insoluble permanent PVA coating on the silica particle surface can be formed simply by dipping silica particles into a hot PVA solution and then settled from this solution, leaving a thin layer of PVA coating and frozen in a freezer. The PVA-Sil shields the silica core from solution erosion to some degree and the pH tolerance range was extended to 9.5 from 8.0 for bare silica. PVA-Sil demonstrated a good hydrophilic property for several kinds of polar compounds and ∼57000 m(-1) of plate count was achieved. This method can also be extended as a universal method to prepare various stationary phases with exchangeable functionalities by doping the desired ingredient in a PVA solution.

MATERIAIS
Número do produto
Marca
Descrição do produto

Sigma-Aldrich
Ácido fórmico, reagent grade, ≥95%
Sigma-Aldrich
Ácido fórmico, ACS reagent, ≥96%
Sigma-Aldrich
Ácido fórmico, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥98%
Sigma-Aldrich
Formiato de amônio, reagent grade, 97%
Sigma-Aldrich
Ácido fórmico, puriss., meets analytical specifications of DAC, FCC, 98.0-100%
Sigma-Aldrich
Formiato de amônio, ≥99.995% trace metals basis
Sigma-Aldrich
Ácido fórmico, ACS reagent, ≥88%
Sigma-Aldrich
Ammonium formate solution, BioUltra, 10 M in H2O
Sigma-Aldrich
Ácido fórmico, ≥95%, FCC, FG
Supelco
Formiato de amônio, eluent additive for LC-MS, LiChropur, ≥99.0%
Sigma-Aldrich
Formic acid solution, BioUltra, 1.0 M in H2O
Sigma-Aldrich
Formiato de amônio, BioUltra, ≥99.0% (calc. based on dry substance, NT)