- Recording SOCE Activity in Neurons by Patch-Clamp Electrophysiology and Microfluorometric Calcium Imaging.
Recording SOCE Activity in Neurons by Patch-Clamp Electrophysiology and Microfluorometric Calcium Imaging.
Methods in molecular biology (Clifton, N.J.) (2018-09-12)
Hsiang-En Wu, Geza Gemes, Quinn H Hogan
PMID30203275
RESUMO
Store-operated Ca2+ entry (SOCE) is a Ca2+ influx pathway at the plasma membrane that replenishes intracellular Ca2+ stores in response to depletion of Ca2+ stores. The SOC current, also known as the Ca2+ release-activated Ca2+ current (ICRAC), has a small conductance, which makes selective recording difficult. This challenge may be addressed using techniques based on identification of Ca2+ influx patch-clamp electrophysiological recording and measurement of cytoplasmic Ca2+ accumulation with Ca2+-sensitive fluorophores. Here, we describe specific methods for studying SOCE using these approaches in rat dorsal root ganglion neurons.
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Desoxirribonuclease I, Type IV, lyophilized powder, ≥2,000 Kunitz units/mg protein
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Trypsin inhibitor from chicken egg white, Type II-O, Partially purified ovomucoid, containing ovoinhibitor
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Tripsina, TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein