Brain inflammation triggers macrophage invasion across the blood-brain barrier in Drosophila during pupal stages.

Brain inflammation triggers macrophage invasion across the blood-brain barrier in Drosophila during pupal stages.

Science advances (2021-10-28)

Bente Winkler, Dominik Funke, Billel Benmimoun, Pauline Spéder, Simone Rey, Mary A Logan, Christian Klämbt

PMID34705495

RESUMO

[Figure: see text].

MATERIAIS

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F4135

Sigma-Aldrich

Soro bovino fetal, USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

A4034

Sigma-Aldrich

L-ascorbato de (+)-sódio, powder, BioReagent, suitable for cell culture

L4655

Sigma-Aldrich

Latex beads, carboxylate-modified polystyrene, fluorescent yellow-green, aqueous suspension, 1.0 μm mean particle size

65906

Sigma-Aldrich

Phalloidin–Atto 647N, BioReagent, suitable for fluorescence, ≥80% (HPLC)

L5530

Sigma-Aldrich

Latex beads, carboxylate-modified polystyrene, fluorescent orange, aqueous suspension, 0.5 μm mean particle size