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Merck
  • In vivo imaging of retrovirus infection reveals a role for Siglec-1/CD169 in multiple routes of transmission.

In vivo imaging of retrovirus infection reveals a role for Siglec-1/CD169 in multiple routes of transmission.

eLife (2021-07-06)
Kelsey A Haugh, Mark S Ladinsky, Irfan Ullah, Helen M Stone, Ruoxi Pi, Alexandre Gilardet, Michael W Grunst, Priti Kumar, Pamela J Bjorkman, Walther Mothes, Pradeep D Uchil
RESUMO

Early events in retrovirus transmission are determined by interactions between incoming viruses and frontline cells near entry sites. Despite their importance for retroviral pathogenesis, very little is known about these events. We developed a bioluminescence imaging (BLI)-guided multiscale imaging approach to study these events in vivo. Engineered murine leukemia reporter viruses allowed us to monitor individual stages of retrovirus life cycle including virus particle flow, virus entry into cells, infection and spread for retroorbital, subcutaneous, and oral routes. BLI permitted temporal tracking of orally administered retroviruses along the gastrointestinal tract as they traversed the lumen through Peyer's patches to reach the draining mesenteric sac. Importantly, capture and acquisition of lymph-, blood-, and milk-borne retroviruses spanning three routes was promoted by a common host factor, the I-type lectin CD169, expressed on sentinel macrophages. These results highlight how retroviruses co-opt the immune surveillance function of tissue-resident sentinel macrophages for establishing infection.

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Roche
DNase I recombinant, RNase-free, from bovine pancreas, expressed in Pichia pastoris
Roche
Dispase® II (neutral protease, grade II), lyophilized, from bacterial, Roche, pkg of 5 × 1 g
Sigma-Aldrich
Gelatina, solid
Sigma-Aldrich
Mix-n-Stain CF 647 Antibody Labeling Kit (50-100μg)
Sigma-Aldrich
Mix-n-Stain CF 488A Antibody Labeling Kit (50-100μg)