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G005922

HPLC Analysis of Aflatoxins B1, B2, G1 and G2 in Corn on Ascentis® Express C18 after SPE using Supel Tox AflaZea and Fluorescence Detection

application for HPLC

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técnica(s)

HPLC: suitable

parâmetros de teste

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: 25 g corn meal in 100 mL acetonitrile:water (84:16)
SPE tube/cartridge: Supel Tox AflaZea, 6 mL (55314-U)
sample addition: 2 mL
elution: collect sample in 5 mL silanized glass vial
eluate post-treatment: transfer 200 μL sample, add 300 μL TFA derivatization solution (70:20:10 water:TFA:acetic acid), heat in microreactor 20 min at 65 °C, cool, add 580 μL deionized water, vortex
column: Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (53825-U)
mobile phase: [A] water; [B] acetonitrile; (80:20, A:B)
flow rate: 0.350 mL/min
pressure: 325 psi (22.4 bar)
column temp.: 35 °C
detector: fluorescence, ex 360 nm, em 440 nm
injection: 20 μL

adequação

application for HPLC

aplicação(ões)

food and beverages

Nota de análise

Aflatoxins are closely related compounds produced as secondary metablolites of fungi. They are found in grains and nuts like corn, wheat or peanuts. Shown here is a rapid, sensitive method to extract these compounds from corn using SPE cartridges specifically designed for aflatoxins, Supel Tox AflaZea (aflatoxin zearalenone), which removes interferences from grains, feeds, TMR samples, peanuts, peanut products and aqueous solutions. Analysis was via HPLC on Ascentis Express Fused-Core C18 colums.

Informações legais

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC

Analito

Descrição

    Aflatoxin G1 solution, 3 μg/mL in benzene:acetonitrile (98:2), analytical standard
    Aflatoxin B1 solution, 20 μg/mL in methanol, analytical standard
    Aflatoxin G2 solution, 3 μg/mL in benzene:acetonitrile (98:2), analytical standard
    Aflatoxin B2 solution, 3 μg/mL in benzene:acetonitrile (98:2), analytical standard

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