567530-U
Discovery® HS F5 (3 µm) HPLC Columns
L × I.D. 5 cm × 4 mm, HPLC Column
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About This Item
Código UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Produtos recomendados
product name
Discovery® HS F5 HPLC Column, 3 μm particle size, L × I.D. 5 cm × 4 mm
Materiais
stainless steel column
Agency
suitable for USP L43
linha de produto
Discovery®
Características
endcapped
fabricante/nome comercial
Discovery®
embalagem
1 ea of
Extensão da rotulagem
12% Carbon loading
Parâmetros
≤70 °C temp. range
400 bar pressure (5801 psi)
técnica(s)
HPLC: suitable
LC/MS: suitable
C × D.I.
5 cm × 4 mm
área da superfície
300 m2/g
cobertura de superfície
4 μmol/m2
Impurezas
<10 ppm metals
matriz
silica gel, high purity, spherical particle platform
fully porous particle
Grupo ativo da matriz
PFP (pentafluorophenyl) phase
tamanho de partícula
3 μm
tamanho de poro
120 Å
operating pH range
2-8
aplicação(ões)
food and beverages
técnica de separação
reversed phase
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Descrição geral
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Características e benefícios
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
Informações legais
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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