ZRB1055

Anti-PGAM5 Antibody, clone 2J4 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Sinônimo(s): Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5, Serine/threonine-protein phosphatase PGAM5 mitochondrial
• Código UNSPSC: 12352203
• NACRES: NA.43

Propriedades

• fonte biológica: rabbit (recombinant)
• Nível de qualidade: 200
• recombinante: expressed in HEK 293 cells
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 2J4, recombinant monoclonal; monoclonal
• linha de produto: ZooMAb^® learn more
• forma: lyophilized
• peso molecular: calculated mol wt 28.02 kDa; calculated mol wt 32 kDa; observed mol wt ~32 kDa; observed mol wt ~34 kDa
• reatividade de espécies: mouse, human
• embalagem: antibody small pack of 25 μL
• características do produto alternativo mais ecológico: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• técnica(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotipo: IgG
• nº de adesão UniProt: Q96HS1
• categoria alternativa mais ecológica: , Aligned
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PGAM5(192111)

Descrição

Descrição geral

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Especificidade

Clone 2J4 is a ZooMAb rabbit recombinant monoclonal antibody that detects both isoforms of human Phosphoglycerate mutase family member 5 (PGAM5). It targets an epitope within 86 amino acids from the N-terminal half.

Imunogênio

His-tagged recombinant fragment corresponding to 86 amino acids from the N-terminal half of human Phosphoglycerate mutase family member 5 (PGAM5).

Aplicação

Anti-PGAM5, clone 2J4 ZooMAb, Cat. No. ZRB1055, is a Rabbit monoclonal antibody that specifically targets PGAM5 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PGAM5 in lysates from HEK293 cells, mouse testis, and human brain tissue.

Immunohistochemistry (Paraffin) Analysis: 1:100 dilution from a representative lot detected PGAM5 in human testis and human cerebral cortex tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound human recombinant PGAM5 with a KD of 3.0 x 10-9 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PGAM5 in MCF7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Descrição-alvo

Serine/threonine-protein phosphatase PGAM5, mitochondrial (UniProt: Q96HS1; also known as EC:3.1.3.16, Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5) is encoded by the pgam5 GENE (Gene ID: 192111) in human. PGAM5 is a single-pass mitochondrial membrane protein that displays phosphatase activity for serine/threonine residues and is shown to dephosphorylate and activate MAP3K5 kinase. It does not display any phosphoglycerate mutase activity. PGAM5 usually exists as a dimer, but can also form ternary complex with Nuclear Factor, Erythroid 2 Like 2 (NFE2L2) and Kelch Like ECH Associated Protein 1 (KEAP1). Its transmembrane domain is localized to amino acids 7 to 29. A 35 amino acid sequence at its N-terminal function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane. Two isoforms of PGAM5 have been described that are produced by alternative splicing. Both isoforms are reported to be phosphorylated by RIPK1/RIPK3 complex under conditions of necrosis and this phosphorylation is shown to increase PGAM5 phosphatase activity. Overexpression of isoform 1 results in clustering of mitochondria around the nucleus whereas isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

forma física

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstituição

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informações legais

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable