U5258

Anti-Ubiquitin C-terminal Hydrolase L1 (RA-15) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinônimo(s): Anti-UCH-L1
• Número MDL: MFCD07370533
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: IgG fraction of antiserum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen 27 kDa
• reatividade de espécies: human, mouse, rat
• técnica(s): microarray: suitable; western blot: 1:1,000-1:2,000 using cytosolic fraction (S1) of mouse brain or whole cell extract of human lung carcinoma A549 cell line; western blot: 1:5,000-1:10,000 using cytosolic fraction (S1) of rat brain
• nº de adesão UniProt: P09936
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... UCHL1(7345) ; mouse ... Uchl1(22223) ; rat ... Uchl1(29545)

Descrição

Descrição geral

Ubiquitin C-terminal hydrolase L1 (UCH-L1) is encoded by the gene mapped to human chromosome 4p13. It is a 223 amino acid deubiquitinating enzyme, expressed highly in neurons.

Imunogênio

synthetic peptide corresponding to amino acids 202-216 located near the C-terminus of rat UCH-L1, conjugated to KLH. This sequence is identical in human, mouse, bovine, porcine, and guinea pig UCH-L1. No homology is found with other known UCH-L isoforms.

Aplicação

Anti-Ubiquitin C-terminal Hydrolase L1 (RA-15) antibody produced in rabbit has been used in immunoblotting.

Ações bioquímicas/fisiológicas

Ubiquitin C-terminal hydrolase L1 (UCH-L1) hydrolyze C-terminal ubiquityl esters and amides in vitro, which is an essential reaction during cytoplasmic protein degradation. peptide-ubiquityl amides are the most favorable substrates. UCH-L1 also acts as a ubiquitin (Ub) ligase. Mutation in the gene is associated with the development of neurodegenerative diseases, such as Parkinson′s disease, spinocerebellar ataxia (SCA) and Huntington′s disease.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)