The amount of material in a given package size varies from lot to lot. The minimum activity of this product is 1.5 units per milligram of protein. The minimum protein concentration is 80%. As an example, a lot reporting an activity of 1.5 units per milligram with an 80% protein concentration will contain 400ug, 800ug, and 4.0 mg of powder in the 1 Unit, 2 Unit, and 10 Unit package sizes, respectively. Please see the link below to review a sample or lot specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/t5398#product-documenation
T5398
Transglutaminase from guinea pig liver
lyophilized powder, ≥1.5 units/mg protein
Sinônimo(s):
Protein-Glutamine-γ-Glutamyltransferase, Protein-glutamine:amine γ-glutamyltransferase
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1 UNIT
R$ 1.568,00
2 UNITS
R$ 2.393,00
10 UNITS
R$ 9.634,00
R$ 1.568,00
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1 UNIT
R$ 1.568,00
2 UNITS
R$ 2.393,00
10 UNITS
R$ 9.634,00
About This Item
Número CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
R$ 1.568,00
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Formulário
lyophilized powder
Nível de qualidade
atividade específica
≥1.5 units/mg protein
peso molecular
76.6 kDa
composição
Protein, ≥80% modified Warburg-Christian
solubilidade
H2O: soluble 1.0 mg/mL, clear
aplicação(ões)
diagnostic assay manufacturing
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
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Aplicação
This product from Sigma has been used to demonstrate that tissue transglutaminase (tTG) selectively deamidates gluten peptides, which results in strongly enhanced T cell-stimulatory activity.[1] It has also been used to assess immune responses to A-gliadin peptides.[2] Furthermore, it has been used to demonstrate that tTG selectively modifies gliadin peptides that are recognized by gut-derived T cells in celiac disease.[3]
Transglutaminase has been used in a study to improve quantifiable assays to fully characterize the role of transglutaminase in diseases such as Huntington′s disease and Alzheimer′s disease.Transglutaminase has also been used in a study to develop a nonradioactive dot blot assay for transglutaminase activity.
Ações bioquímicas/fisiológicas
Transglutaminase from guinea pig liver consists of a single polypeptide chain of 691 amino acid residues. It has six potential glycosylation sites (Asn-X-Ser or Asn-X-Thr), but it is not glycosylated. The molecular mass is approximately 76.6 kDa.[4] It is calcium dependent and has several calcium binding sites. The enzyme is inhibited by iodoacetamide and N-ethylmaleimide in the presence of calcium.[5] It catalyzes the incorporation of small molecular weight amines into γ-glutamine sites of proteins. In the absence of small molecular weight amines, it catalyzes the cross linking of proteins that results in the formation of γ-glutamyl-ε-lysine side chain peptides. Liver transglutaminase is a nonzymogenic enzyme.[6]
Definição da unidade
One unit will catalyze the formation of 1.0 μmole of hydroxamate per min from Nα-Z-Gln-Gly and hydroxylamine at pH 6.0 at 37 °C. (L-Glutamic acid γ-monohydroxamate is the standard.)
forma física
Lyophilized powder containing Tris and dithioerythritol
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
Eyeshields, Gloves, type N95 (US)
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Y van de Wal et al.
Journal of immunology (Baltimore, Md. : 1950), 161(4), 1585-1588 (1998-08-26)
Celiac disease (CD) is caused by gluten ingestion in susceptible individuals. Tissue transglutaminase (tTG)-specific Abs are characteristic of CD, and increased tTG activity has been observed in the jejunal biopsies of patients. Here we demonstrate that tTG selectively deamidates gluten
Jessica C Kiefte-de Jong et al.
Gastroenterology, 144(4), 726-735 (2013-01-15)
Celiac disease in pregnant women has been associated with poor growth of the fetus, but little is known about how the level of celiac disease affects fetal growth or birth outcomes. We assessed the associations between levels of antibodies against
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Breast cancer is the most common malignancy among women. With the aim of decreasing the toxicity of conventional breast cancer treatments, an alternative that could provide appropriate and effective drug utilization was envisioned. Thus, we contemplated and compared the in
L Lorand et al.
Molecular and cellular biochemistry, 58(1-2), 9-35 (1984-01-01)
This paper is intended as a background to the topic of transglutaminases, while focusing on current ideas regarding the biological roles of these enzymes. Specifically, the following topics are discussed: geometry of forming gamma-glutamyl-epsilon-lysine cross-linked structures; energetic considerations; the gamma-glutamyl-epsilon-lysine
Mechanism of action of guinea pig liver transglutaminase. I. Purification and properties of the enzyme: identification of a functional cysteine essential for activity.
J E Folk et al.
The Journal of biological chemistry, 241(23), 5518-5525 (1966-12-10)
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Hello , how much grams are in one Unit Transglutaminase enzyme T5398 ? thanks very much kind regards Alex
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