SRP6057

Activin A from mouse

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Sinônimo(s): FRP, FSH-releasing protein, Inhibin beta-1
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: mouse
• recombinante: expressed in E. coli
• Ensaio: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Formulário: lyophilized
• peso molecular: 26.2 kDa
• embalagem: pkg of 10 μg
• técnica(s): cell culture | embryo: suitable
• nº de adesão NCBI: P08476
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: mouse ... INHBA(3624)

Descrição

Descrição geral

Activin A is a member of the TGF (transforming growth factor)-β family of proteins produced by many cell types throughout development. Mouse Activin A has 100% amino acid sequence identity to human, rat, porcine, bovine and feline proteins. Recombinant mouse Activin A is a nonglycosylated homodimer, containing two 117 amino acid chains, with a total molecular weight of 26.2 kDa.[1][2]

Aplicação

Activin A has been used:

• to induce differentiation of embryonic stem cells to pancreatic β cells[3]
• in the induction of bone marrow-derived mesenchymal stem cells to insulin-producing cells[4]
• to induce endoderm differentiation from human embryonic stem cells[5]
• as a component of β cell induction medium. [6]

Ações bioquímicas/fisiológicas

Activins interact with Type I and Type II serine/threonine kinases to signal to SMAD proteins to regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, metabolism, etc. Activin A is a homodimer of two β A chains and is not biologically active until the N terminal propeptide is cleaved from each chain.[1][2][7] Activin A is involved in mesoderm formation and hematopoietic development.

forma física

Recombinant mouse Activin A is lyophilized with 0.02% TFA.

Nota de preparo

Centrifuge the vial prior to opening.

Reconstituição

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water, which can then be further diluted to other aqueous solutions.

Outras notas

MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H315,H319
• Declarações de precaução: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Classificações de perigo: Eye Irrit. 2 - Skin Irrit. 2
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable