SRP5348
GST protein, tag-free
GST protein, tag-free
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
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20 μG
R$ 583,00
About This Item
Produtos recomendados
recombinante
expressed in E. coli
Ensaio
≥70% (SDS-PAGE)
Formulário
buffered aqueous glycerol solution
peso molecular
~25 kDa
técnica(s)
ligand binding assay: suitable
nº de adesão NCBI
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Informações sobre genes
Escherichia coli IAI39 ... gst> gst
gst(7152360)
Descrição geral
Glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial and microsomal proteins that are used to create the so-called GST gene fusion system. These proteins are capable of multiple reactions with a multitude of substrates. They have a role in detoxication and toxification mechanisms.[1] GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small. GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione.
Aplicação
forma física
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Nota de preparo
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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