SRP3296

WNT-7A human

recombinant, expressed in HEK 293 cells, ≥80% (SDS-PAGE), ≥80% (HPLC)

• Sinônimo(s): Human WNT-7A, Recombinant WNT-7A, WNT-7A Signaling Molecule
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in HEK 293 cells
• Ensaio: ≥80% (HPLC); ≥80% (SDS-PAGE)
• forma: lyophilized
• potência: 40-60 ng/mL ED₅₀
• peso molecular: 35.5 kDa
• embalagem: pkg of 15 μg
• Impurezas: <0.1 EU/μg endotoxin, tested
• cor: white
• nº de adesão UniProt: O00755
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• Informações sobre genes: human ... WNT7A(7476)

Descrição

Descrição geral

Wnt-7a is expressed in placenta, kidney, testis, uterus, fetal lung and fetal and adult brain. Most Wnt proteins can signal though a mechanism called the canonical Wnt pathway, in which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors and induces the expression of numerous genes. Increased Wnt/β-Catenein signaling is associated with tumorigenesis in a diverse set of human cancers. However, Wnt-7a/Frizzled-9 signaling has been shown to act as a tumor suppressor in non-small cell lung cancers.

Ações bioquímicas/fisiológicas

Wnt-7a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. Recombinant human Wnt-7a is a 35.5 kDa glycoprotein containing 318 amino acids. Due to glycosylation, Wnt-7a migrates between 40-55 kDa by SDS-PAGE gel under unreduced conditions

forma física

Lyophilized with no additives.

Reconstituição

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of ≤0.2 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable