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Documentos Principais

SRP2096

Sigma-Aldrich

p300-Ch1 (302-531) human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sinônimo(s):

KAT3B, RP1-85F18.1, RSTS2, p300

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.26

fonte biológica

human

recombinante

expressed in E. coli

Ensaio

≥80% (SDS-PAGE)

Formulário

frozen liquid

peso molecular

~26 kDa

embalagem

pkg of 10 μg

concentração

1000 μg/mL

cor

clear colorless

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Informações sobre genes

human ... EP300(2033)

Descrição geral

The EP300 (E1A binding protein p300) gene is mapped to human chromosome 22q13.2.[1] It codes for a histone acetyltransferase which is ubiquitously expressed.[2]

Ações bioquímicas/fisiológicas

Human p300 and CBP (CREB binding protein) are highly related transcriptional coactivators. Both proteins have been identified through protein interaction assays. In addition to interacting with variety of cellular factors and onco-proteins, loss of the wild type CBP alleles in isolated tumors suggests that CBP/p300 might serve as tumor suppressors. The ability of p300 to acetylate many transcription factors, including p53, E2F, TFIIE, and TFIIF etc. demonstrated a novel mechanism of targeted p300 regulation of gene expression.
Loss of EP300 (E1A binding protein p300) gene function causes Rubinstein-Taybi syndrome.[3] p300 is essential for cell differentiation, growth and survival.[2] p300 promotes liver stiffness, which is known to induce hepatic stellate cell differentiation to tumor-promoting myofibroblasts.[4]

forma física

Clear and colorless frozen liquid solution

Nota de preparo

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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P300 acetyltransferase mediates stiffness-induced activation of hepatic stellate cells into tumor-promoting myofibroblasts
Dou C, et al.
Gastroenterology, 154(8), 2209-2221 (2018)
Genome-wide sequencing expands the phenotypic spectrum of EP300 variants
Costain G, et al.
European Journal of Medical Genetics, 61(3), 125-129 (2018)
Dysregulation of Sirtuin 2 (SIRT2) and histone H3K18 acetylation pathways associates with adverse prostate cancer outcomes
Damodaran S, et al.
BMC Cancer, 17(1), 874-874 (2017)
Jeroen H Roelfsema et al.
American journal of human genetics, 76(4), 572-580 (2005-02-12)
CREB-binding protein and p300 function as transcriptional coactivators in the regulation of gene expression through various signal-transduction pathways. Both are potent histone acetyl transferases. A certain level of CREB-binding protein is essential for normal development, since inactivation of one allele
R W Stein et al.
Journal of virology, 64(9), 4421-4427 (1990-09-01)
Adenovirus E1A transforming function requires two distinct regions of the protein. Transforming activity is closely linked with the presence of a region designated conserved domain 2 and the ability of this region to bind the product of the cellular retinoblastoma

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