SRP0415

PKM1 active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Sinônimo(s): OIP3, PK3, PKM2, TCB, pyruvate kinase, muscle
• Número CAS: 1644286-11-2
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in E. coli
• Ensaio: ≥80% (SDS-PAGE)
• Formulário: aqueous solution
• peso molecular: 59 kDa
• embalagem: pkg of 20 μg
• fabricante/nome comercial: Sigma-Aldrich
• condição de armazenamento: avoid repeated freeze/thaw cycles
• concentração: 1.8 mg/mL
• técnica(s): cell based assay: suitable
• solubilidade: water: soluble
• nº de adesão NCBI: NM_182470
• nº de adesão UniProt: P14618
• aplicação(ões): life science and biopharma
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... PKM(5315)

Descrição

Descrição geral

Research area: Cell Signaling

Pyruvate kinase (PK) has four isozymes such as PKL, PKR, PKM1, and PKM2.[1][2] PKM1/2 is located in the distal convoluted tubule of the kidney, embryo, lungs, proliferative tissues,[2] retina, adipose tissues, and islets.[1] The PKM1 gene is located on the human chromosome at 15q23.[3]

Aplicação

PKM1active human has been used in:

• cell-based kinase assay to study the ability of a compound to inhibit the kinase activity of PKM1.[4]
• oxidative stress experiments on PKM2.[5]
• Co-immunoprecipitation (Co-IP) to determine pyruvate kinase M2 (PKM2) as a novel direct binding partner of phosphoserine aminotransferase 1 (PSAT1).[6]

Ações bioquímicas/fisiológicas

Pyruvate kinase (PK) catalyzes the dephosphorylation of phosphoenolpyruvate(PEP) into pyruvate. It is also involved in glycolysis by releasing ATP.Allosteric effects and intracellular signal transduction regulate the enzymaticactivity of PKM1. PKM1 is involved in several biological processes such asmetabolic reprogramming, and innate and acquired immune response. Itparticipates in protein interactions, protein synthesis, glycolysis, andintranuclear signal transduction.[1]

Informações de segurança

• Pictogramas: GHS08,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H315,H319,H360D
• Declarações de precaução: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classificações de perigo: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Código de classe de armazenamento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable