SRP0235

PI3 kinase [p110a(H1047R)/p85a] Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

• Sinônimo(s): PIK3CA, Phosphoinositide-3-kinase, catalytic α, PtdIns-3-kinase p110
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in baculovirus infected insect cells
• Ensaio: ≥60% (SDS-PAGE)
• forma: aqueous solution
• atividade específica: ≥1770 pmol/min-μg
• peso molecular: 129 kDa (p110α); 88 kDa (p85α)
• embalagem: pkg of 20 μg
• concentração: 0.38 mg/mL
• nº de adesão NCBI: U79143 (p110alpha); XM_043865 (p85alpha)
• nº de adesão UniProt: P42336
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... PIK3CA(5290)

Descrição

Descrição geral

Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. U79143), with H1047R mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.

Aplicação

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definição da unidade

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Nota de preparo

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2