TEV protease is a highly sequence specific serine protease from Tobacco Etch Virus. Its high specificity makes TEV protease popular for cleaving recombinant fusion proteins. However, at low temperatures such as 4 °C, standard TEV Protease has only ~10% of its activity compared to regular temperature conditions (such as 25-30 °C). IceTEV Protease is a new TEV Protease version that is designed to cleave fusion proteins effectively at low temperatures also, in the range of 2-10 °C, with comparable efficiency to cleavage by standard TEV Protease at 25-30 °C. Following the cleavage reaction, IceTEV Protease can be removed from the reaction mixture with nickel-agarose (Ni-agarose) beads.
Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.
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