SAB4200366

Anti-IKZF1/IKAROS antibody, Mouse monoclonal

clone E1.14, purified from hybridoma cell culture

• Sinônimo(s): Anti-Hs.54452, Anti-IK1, Anti-IKAROS family zinc finger 1, Anti-LYF1, Anti-PRO0758, Anti-ZNFN1A1, Anti-hIk-1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: E1.14, monoclonal
• forma: buffered aqueous solution
• peso molecular: antigen ~55 kDa
• reatividade de espécies: rat, monkey, human, mouse
• concentração: ~1.0 mg/mL
• técnica(s): flow cytometry: suitable; immunocytochemistry: 0.25-0.5 μg/mL using Jurkat cells; indirect immunofluorescence: suitable; western blot: 0.25-0.5 μg/mL using Ramos cells
• Isotipo: IgG2b
• nº de adesão UniProt: Q13422
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... IKZF1(10320)

Descrição

Descrição geral

Monoclonal Anti- IKZF1 (Ikaros family zinc finger protein 1)/IKAROS (mouse IgG2b isotype) is derived from the hybridoma E1.14 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide located at the C-terminus human IKZF1/IKAROS. The IKAROS gene encodes a family of hemopoietic specific zinc finger transcriptional regulator proteins, which binds to specific DNA target sequences consisting of TGGGAA consensus motif.

Imunogênio

a peptide located at the C-terminus human IKZF1/IKAROS.

Aplicação

Monoclonal Anti-IKZF1 (Ikaros family zinc finger protein 1)/IKAROS antibody produced in mouse has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry
• flow cytometry

Ações bioquímicas/fisiológicas

Ikaros family zinc finger protein 1 (IKZF1)/IKAROS activity is essential for the generation of B cell precursors. Ikaros is essential at the earliest stage of T cell and B cell specification. In adults, however, lymphoid lineages rely on Ikaros at distinct phases of their development. Mutations or polymorphisms are associated with human B-cell acute lymphoblastic leukemia (B-ALL). Ikaros functions as a tumor suppressor in T-cell acute lymphoblastic leukemia (T-ALL) and B-ALL. Ikaros deletion was detected in patients with blast-phase myeloproliferative neoplasms.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable