SAB4200048

Anti-E1B-AP5 antibody, Rat monoclonal

clone EAP4A11, purified from hybridoma cell culture

• Sinônimo(s): Anti-Adenovirus E1B 55-KD protein-associated protein 5, Anti-Heterogeneous nuclear ribonucleoprotein U-like 1, Anti-hnRNPUL1, Anti-hnRPUL1, Monoclonal Anti-E1B-AP5 antibody produced in rat
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rat
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: EAP4A11, monoclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~110 kDa
• reatividade de espécies: bovine, canine, human, rat, monkey, mouse
• técnica(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.05-0.1 μg/mL using HT-29 cell extracts
• Isotipo: IgG2b
• nº de adesão UniProt: Q9BUJ2
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... HNRNPUL1(11100)

Descrição

Descrição geral

Monoclonal Anti-E1B-AP5 (rat IgG2b isotype) is derived from the hybridoma EAP4A11 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with full length human E1B-AP5 fusion protein. E1B-55K-associated protein 5 (E1B-AP5), also known as heterogeneous nuclear ribonucleoprotein U-like 1(hnRNPUL1), is a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family.

The E1B-AP5 gene is located on the human chromosome at 19q13.2.

Especificidade

Monoclonal Anti-E1B-AP5 recognizes human, monkey, bovine, dog, mouse and rat E1B-AP5.

Imunogênio

full length human E1B-AP5 fusion protein.

Aplicação

Monoclonal Anti-E1B-AP5 antibody produced in rat may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry

Ações bioquímicas/fisiológicas

E1B-55K-associated protein 5 (E1B-AP5) plays a vital role in ATR signaling. In addition, it may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B55kDa in adenovirus-infected cells. Interestingly, this protein was found to be associated with bromodomain-containing protein, BRD7 in a complex that links chromatin events with mRNA processing at the level of transcriptional regulation. It also activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation.

forma física

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable