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RAB0242

Sigma-Aldrich

Human IgG1 ELISA Kit

for serum, plasma, conditioned medium, urine

Sinônimo(s):

IgG1, Immunoglobulin G1

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.32

reatividade de espécies

human

embalagem

kit of 96 wells (12 strips x 8 wells)

técnica(s)

ELISA: suitable
capture ELISA: suitable

entrada

sample type plasma
sample type serum
sample type conditioned medium
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 0.05-40 ng/mL

método de detecção

colorimetric

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Informações sobre genes

human ... IGHG1(3500)

Descrição geral

The Human IgG1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IgG1 in serum and plasma.

Imunogênio

Recombinant Human IGG1

Aplicação

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Ações bioquímicas/fisiológicas

Immunoglobulin heavy constant gamma 1 (IGHG1), or IgG1, expressed in pancreatic cancer cells might be implicated in tumor cell proliferation and immune evasion mechanisms. siRNA targeted silencing of IGHG1 retards cell growth and induces apoptosis in human prostate cancer cell.

Outras notas

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Componentes do kit também disponíveis separadamente

Nº do produto
Descrição
SDS

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictogramas

Corrosion

Palavra indicadora

Warning

Frases de perigo

Declarações de precaução

Classificações de perigo

Met. Corr. 1

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials


Certificados de análise (COA)

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Roxanne Collin et al.
Journal of immunology (Baltimore, Md. : 1950), 193(7), 3503-3512 (2014-08-29)
Autoimmune diseases result from a break in immune tolerance. Various mechanisms of peripheral tolerance can protect against autoimmunity, including immunoregulatory CD4(-)CD8(-) double-negative (DN) T cells. Indeed, we have previously shown that diabetes-prone mouse strains exhibit a low proportion of DN
Bin Pan et al.
Molecular biology reports, 40(1), 27-33 (2012-11-03)
To investigate the immunoglobulin G (IgG) expression in prostate cancer cell lines and explore the effects of IGHG1 gene knockdown on PC3 cell growth and apoptosis. Flow cytometry, qPCR and western blot were used to demonstrate IgG expression in prostate
Xiaoyan Li et al.
Pancreas, 40(5), 753-761 (2011-06-10)
To investigate the expression of Igγ-1 chain C region (IGHG1) in human pancreatic carcinomas and determine the biological function of IGHG1 expression in immune evasion mechanisms. Comparative proteomic analysis was used to detect the differential expression of IGHG1 in human
Joseph R Brady et al.
Biotechnology and bioengineering, 117(2), 543-555 (2019-10-28)
Komagataella phaffii, also known as Pichia pastoris, is a common host for the production of biologics and enzymes, due to fast growth, high productivity, and advancements in host engineering. Several K. phaffii variants are commonly used as interchangeable base strains
Arjan van der Flier et al.
PloS one, 10(4), e0124930-e0124930 (2015-04-24)
We recently developed a longer lasting recombinant factor VIII-Fc fusion protein, rFVIIIFc, to extend the half-life of replacement FVIII for the treatment of people with hemophilia A. In order to elucidate the biological mechanism for the elongated half-life of rFVIIIFc

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