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PCR9604

Sigma-Aldrich

GenElute™ 96 Well PCR Clean-Up Kit

4 x 96 well plate, sufficient for 4 96-well plate purifications

Sinônimo(s):

High Throughput Purification, PCR Purification, PCR Purification, PCR Purification Kit

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About This Item

Código UNSPSC:
41106300
NACRES:
NA.52

uso

sufficient for 4 96-well plate purifications

qualidade

4 x 96 well plate

técnica(s)

DNA purification: suitable

temperatura de armazenamento

15-25°C

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Descrição geral

GenElute 96 Well PCR Clean-Up Kit allows for high throughput purification of PCR products. The kit provides the necessary reagents for purification of highly pure PCR products. The DNA recovery is 75-90% for fragments of 100 bp to 10 kb and removes primers, primer-dimers, nucleotides, salts and polymerase.

Once the PCR reaction is complete, the reaction volume is adjusted and Binding Solution is added. The samples are transferred to the Binding Plate where the PCR reaction is captured on the silica membrane. The bound PCR product is washed several times to remove primers, primer-dimers, salts, nucleotides, and polymerases. Finally the purified PCR product is eluted and ready for immediate use in downstream applications.

The purified PCR product is ready for a variety of downstream applications including sequencing, restriction digest, ligation, and microarray analysis.
GenElute 96 Well PCR Clean-Up Kit allows for high throughput purification of PCR products. The kit provides the necessary reagents for purification of highly pure PCR products. The DNA recovery is 75-90% for fragments of 100 to 10,000 bp and removes primers, primer-dimers, nucleotides, salts and polymerase.

Aplicação

The purified PCR product is ready for a variety of downstream applications including sequencing, restriction digest, ligation and microarray analysis.

Características e benefícios

  • Complete removal of primers and primer-dimers
  • Innovative wash plate minimizes risk of cross-contamination
  • Suitable for processing under vacuum and centrifugation
  • Time-saving parallel clean-up of PCR products

Princípio

Once the PCR reaction is complete, the reaction volume is adjusted and the Binding Solution is added. The samples are transferred to the Binding Plate where the PCR reaction is captured on the silica membrane. The bound PCR product is washed several times to remove primers, primer-dimers, salts, nucleotides and polymerases. Finally the purified PCR product is eluted and ready for immediate use in downstream applications.

Informações legais

GenElute is a trademark of Sigma-Aldrich Co. LLC

Pictogramas

CorrosionExclamation mark
GHS05,GHS07

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Perigos de suplementos

EUH032

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

Classe de risco de água (WGK)

WGK 3

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Certificados de análise (COA)

Lot/Batch Number
MKCP8788
071M0752

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