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Documentos Principais

P7899

Sigma-Aldrich

Anti-Peroxidase antibody produced in rabbit

fractionated antiserum, lyophilized powder

Sinônimo(s):

Rabbit Anti HRP

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

rabbit

Nível de qualidade

conjugado

unconjugated

forma do anticorpo

fractionated antiserum

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

lyophilized powder

reatividade de espécies

horseradish

técnica(s)

indirect ELISA: 1:100,000-1:200,000

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Descrição geral

Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
Peroxidase is a hemoprotein.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.

Imunogênio

Purified peroxidase from horseradish

Aplicação

Anti-Peroxidase antibody produced in rabbit has been used in:
  • immunoblot assays
  • enzyme-linked-immunosorbent assay (ELISA)
  • immunoprecipitation

Ações bioquímicas/fisiológicas

Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.

forma física

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2

Reconstituição

Reconstitute with deionized water.

Nota de análise

Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Sigma-Aldrich

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