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MX568S100

Sigma-Aldrich

Mix-n-Stain CF 568 Antibody Labeling Kit (50-100μg)

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

forma

liquid

Nível de qualidade

condição de armazenamento

protect from light

fluorescência

λex 562 nm; λem 583 nm

temperatura de armazenamento

−20°C

Descrição geral

This labeling kit is for labeling 50-100 μg antibody with the red fluorescent dye CF568. Mix-n-Stain CF dye antibody labeling kits are a revolutionary antibody labeling technology that permits one to label antibodies with our superior CF dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. CF568 is a red fluorescent dye optimally excitable by the 561 nm or the 568 nm laser line. Antibody conjugates of the dye are much brighter than those of Alexa Fluor 568. In addition, the photostability of CF568 is superior to that of Alexa Fluor 568, making CF568 a much better choice for demanding applications, such as confocal microscopy and single molecular imaging.

Aplicação

The Mix-n-Stain CF dye antibody labeling kit can be used to label primary antibody for ELISA, western blot, immunohistochemistry, flow cytometry, immunofluorescence or immunocytochemistry applications.

Características e benefícios

A major advantage of the labeling kits is the superior CF dyes, many of which are brighter and more photostable than Alexa Fluor dyes and other similar commercial dyes. With a large list of CF dyes of wavelengths covering the entire visible and near-infrared spectra, you have unprecedented freedom to label any commercial primary antibody with a color of your choice, making multi-color detection simple and affordable.
Mix-n-Stain CF dye antibody labeling kits dramatically simplify the process of preparing fluorescently labeled antibodies, particularly primary antibodies. Simply mix your antibody with a CF dye of your choice in the buffer provided, a step that takes less than 30 seconds of hands-on time. After waiting for another 30 minutes, without a separation step, you will have a fluorescent antibody conjugate that is as good as the best pre-labeled fluorescent antibody you can buy from some of the leading suppliers. There is no need to calculate how much dye you should use; just mix your antibody with the entire amount of dye provided and you will always produce optimal labeling. Moreover, the labeling reaction can tolerate the presence of common stabilizers, such as sodium azide, Tris, glycerol and low level of BSA.

Componentes

The kit consists of 1 vial each of Mix-n-Stain Dye, Mix-n-Stain Reaction Buffer (10x), Mix-n-Stain Storage Buffer, and an ultrafiltration vial.

Nota de análise

Direct replacement for: Alexa Fluor 568, ATTO 565, DY-560, Rhodamine Red

Informações legais

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
CF is a trademark of Biotium, Inc.
Mix-n-Stain is a trademark of Biotium, Inc.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogramas

Corrosion

Palavra indicadora

Warning

Frases de perigo

Declarações de precaução

Classificações de perigo

Aquatic Chronic 3 - Met. Corr. 1

Código de classe de armazenamento

8A - Combustible corrosive hazardous materials

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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