MAK206
Fumarase Activity Colorimetric Assay Kit
Sufficient for 100 Colorimetric tests
Sinônimo(s):
Fumarate Hydratase Activity Colorimetric Assay Kit
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.84
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Descrição geral
The new Fumarase Assay Kit, MAK521, is now available! Fumarase (Fumarate Hydratase; EC 4.2.1.2) catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (Krebs) cycle. The activity of fumarase is important as it produces reducing equivalents that drive the synthesis of ATP and maintains energy balance in aerobic metabolism. A deficiency of fumarase activity results in progressive encephalopathy.
Aplicação
Fumarase Activity Colorimetric Assay Kit has been used to determine fumarase (FUM) activity.
Adequação
Suitable for the detection of Fumarase in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
Princípio
The Fumarase Activity Colorimetric Assay Kit provides a simple and high-throughput ready procedure for measuring fumarase activity in a variety of tissues, cells, and isolated mitochondria. Fumarase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of fumarase is the amount of enzyme that generates 1.0 mmole of NADH per minute at pH 9.5 at 37 °C.
Somente componentes do kit
Nº do produto
Descrição
- Fumarase Assay Buffer
- Fumarase Substrate
- Fumarase Enzyme Mix
- Fumarase Developer
- NADH Standard
- Fumarase Positive Control
substituído por
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Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Dam. 1 - Skin Corr. 1B
Código de classe de armazenamento
8A - Combustible corrosive hazardous materials
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