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MAK075
Ascorbic Acid Assay Kit II
sufficient for 100 colorimetric tests
Sinônimo(s):
FRASC Assay, Ferric Reducing/Antioxidant and Ascorbic Acid
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Produtos recomendados
uso
sufficient for 100 colorimetric tests
método de detecção
colorimetric
temperatura de armazenamento
−20°C
Descrição geral
The new Ascorbic Acid Assay Kit, MAK505, is now available! Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.
Aplicação
Ascorbic Acid Assay Kit II has been used to determine ascorbic acid concentration in vitreous sample from the eye.[1]
Adequação
Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.
Princípio
In this assay, ascorbic acid concentration is determined using the Ferric Reducing/Antioxidant and Ascorbic Acid (FRASC) assay. Fe3+ is reduced to Fe2+ by antioxidants present in the sample, which results in a colorimetric (593 nm) product. The addition of ascorbate oxidase to parallel samples oxidizes any ascorbic acid present allowing for the measurement of the ascorbic acid concentration.
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10 - Combustible liquids
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Karthik Murali et al.
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We investigated the spatial variation of vitreous oxygen consumption in enucleated porcine eyes. A custom made oxygen source was fabricated that could be localized to either the mid or posterior vitreous cavity and steady state vitreous oxygen tension was measured
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Can product MAK075, Ascorbic Acid Assay kit, be used with tissue samples which have been frozen overnight?
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As long as the tissue is frozen in liquid nitrogen and stored at -80 °C, it should be fine. Since ascorbate is easiliy vulnerable to oxidation, there could be some differences between the assayed values in fresh and frozen samples.
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