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MAK505

Sigma-Aldrich

Ascorbic Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84
Preço e disponibilidade não estão disponíveis no momento.

Nível de qualidade

aplicação(ões)

food and beverages
pharmaceutical

método de detecção

colorimetric
fluorometric

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

Descrição geral

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damage.

Características e benefícios

  • Linear detection range: 6 to 1000 µM for the colorimetric assay and 1 to 100 µM for the fluorometric assay.

Adequação

The kit is suitable for ascorbic acid determination in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture, as well as for studying the effects of drugs on ascorbic acid metabolism.

Princípio

The Ascorbic Acid Assay Kit provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the ascorbic acid concentration in the sample.

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2


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Questions

  1. Can this kit be used to detect Ascorbic acid in rat plasma/serum

    1 answer
    1. This kit reacts with mammals in general and may be used with samples from rats. The kit is suitable for ascorbic acid determination in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture, as well as for studying the effects of drugs on ascorbic acid metabolism.

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