L7025

LB Broth with agar (Lennox)

Tablet microbial growth medium

• Sinônimo(s): LB Agar, LB agar
• Código UNSPSC: 12352200
• NACRES: NA.85

Propriedades

• grau: for molecular biology
• esterilidade: non-sterile
• forma: tablet
• técnica(s): microbiological culture: suitable
• aplicação(ões): food and beverages; microbiology
• temperatura de armazenamento: room temp
• adequação: nonselective for Escherichia coli; nonselective for coliforms

Descrição

Descrição geral

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Aplicação

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Características e benefícios

Lennox LB tablet with agar provides:

• A budget-friendly alternative to pre-poured plates
• Convenient small package to eliminate weighing
• Fast preparation – no stirring required

Componentes

13.72g/L Agar
9.14g/L Tryptone
4.57 g/L Yeast Extract
4.57 g/L NaCl
1.6g/L inert binding agents

Reconstituição

Add each 1.68 g tablet to 48.3 mL water (no stirring required). Autoclave for 20 minutes at 121 °C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Informações legais

Zeocin is a registered trademark of Cayla Sarl

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable