L1378

L-Lactic Dehydrogenase from bovine muscle

Type X, ammonium sulfate suspension, ≥600 units/mg protein

• Sinônimo(s): (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD, Lactate
• Número CAS: 9001-60-9
• Número da licença da enzima: 1.1.1.27 (BRENDA, IUBMB)
• Número CE: 232-617-8
• Número MDL: MFCD00131466
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• fonte biológica: bovine muscle
• tipo: Type X
• Ensaio: 3.0—25.0 mg protein/mL (biuret)
• forma: ammonium sulfate suspension
• atividade específica: ≥600 units/mg protein
• fabricante/nome comercial: Sigma-Aldrich
• técnica(s): activity assay: suitable
• cor: white
• pH: 7.5
• nº de adesão UniProt: P19858; Q5E9B1
• aplicação(ões): life science and biopharma
• atividade externa: pyruvate kinase ≤0.01%
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: cow ... LDHA(281274) , LDHB(281275)

Descrição

Descrição geral

Research Area: Cell Signaling
Lactate dehydrogenase (LDH) is a key enzyme in the anaerobic metabolic pathway and falls under the class of oxidoreductases. This enzyme is found in a wide range of organisms, including both plants and animals. It is widely distributed across all tissues. LDH is primarily a cytoplasmic enzyme, with particularly high concentrations in muscle, liver, and kidney tissues.

Aplicação

L-Lactic Dehydrogenase from bovine muscle has been used in lactate assay to assess lactate tissue accumulation.

Ações bioquímicas/fisiológicas

Lactate dehydrogenase (LDH) plays a critical role as a checkpoint in gluconeogenesis and DNA metabolism. It plays a vital role in maintaining homeostasis during periods of low oxygen availability. As an oxidoreductase enzyme, lactate dehydrogenase catalyzes the reversible conversion of pyruvate to lactate using NADH. This enzyme is crucial for the anaerobic metabolism of glucose when oxygen is either absent or in short supply. LDH is the sole serum biomarker effective for evaluating metastatic melanomas.

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Definição da unidade

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

forma física

Crystalline suspension in 2.4 M (NH₄)₂SO₄ solution, pH 6.0

Nota de análise

Protein determined by biuret.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)