H0273
Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse
clone IAP-28, ascites fluid
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About This Item
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
unconjugated
forma do anticorpo
ascites fluid
tipo de produto de anticorpo
primary antibodies
clone
IAP-28, monoclonal
peso molecular
antigen 25 kDa
contém
15 mM sodium azide
reatividade de espécies
chicken, bovine, frog, rabbit, guinea pig, turkey, Drosophila, mouse, rat, human
técnica(s)
immunohistochemistry: suitable using frozen sections of chicken gizzard smooth muscle
indirect ELISA: suitable
indirect immunofluorescence: suitable using formalin-fixed, heat-shocked cultured chick embryo fibroblasts
radioimmunoassay: suitable
western blot: 1:500 using turkey gizzard extract
Isotipo
IgG1
nº de adesão UniProt
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... HSPB1(3315)
mouse ... Hspb1(15507)
rat ... Hspb1(24471)
Descrição geral
Monoclonal anti-Heat Shock Protein 25 (mouse IgG1) isotype) is derived from the IAP-28 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle. Heat shock (stress) proteins (HSPs) are a group of polypeptides synthesized in response to a wide variety of environmental factors, including biotic and abiotic stressors. These proteins are produced by prokaryotic and eukaryotic cells, and HSPs are among the most conserved molecules in phylogeny. Heat shock protein 25 is encoded by the gene mapped to human chromosome 7q11.23. The encoded protein belongs to the class of small HSPs. Small HSPs aggregate to form characteristic ring-shaped structures called heat shock granules. Small HSPs contains a-crystallin domain” (ACD) C-terminal domain, N-terminal domain and C-terminal extension (CTE).
Especificidade
The antibody also reacts with a dimer of the HSP 25 (approx. 58 kDa) appearing in preparations stored for prolonged periods prior to electrophoretic analysis. A major proteolytic fragment of 22 kDa may also be detected. The antibody suppresses the inhibition of actin elongation induced by the 25 kDa IAP. The antibody displays a punctate or fine fibrillary pattern of labeling throughout the cytoplasm in heat-shocked cells and co-localization with mitochondria. Reactivity has been observed with HSP 25 of various organs.
Imunogênio
partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.
Aplicação
Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse has been used in:
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- radioimmunoassay (RIA)
- immunocytochemistry
- immunohistofluorescence assay
- immunopurification
Ações bioquímicas/fisiológicas
Small heat shock proteins (HSPs) play a vital role in stress tolerance. They act as molecular chaperones in vitro. Murine Hsp25 averts aggregation of citrate synthase and α-glucosidase. It also inhibits actin polymerization and the amyloid fibril formation of Aβ1-42. Mutations in human sHsps result in myopathies, neuropathies and cataract. The small HSPs have also been implicated in phenomena of acquired thermotolerance and neoplasia.
forma física
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
Armazenamento e estabilidade
For continuous use, store at 2-8 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
nwg
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Expression of Heat Shock Protein (HSP)-25 and HSP-32 in the Rat Spinal Cord Reconstructed with Neurogel TM
Woerly S, et al.
Neurochemical Research, 30(6-7) (2005)
Heat shock protein expression in fish
Iwama GK, et al.
Reviews in Fish Biology and Fisheries, 8(1), 35-56 (1998)
Small heat shock proteins: role in cellular functions and pathology
Bakthisaran R, et al.
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1854(4), 291-319 (2015)
Human alpha B-crystallin mutation causes oxido-reductive stress and protein aggregation cardiomyopathy in mice
Rajasekaran NS, et al.
Cell, 130(3), 427-439 (2007)
Constantin d'Ydewalle et al.
Nature medicine, 17(8), 968-974 (2011-07-26)
Charcot-Marie-Tooth disease (CMT) is the most common inherited disorder of the peripheral nervous system. Mutations in the 27-kDa small heat-shock protein gene (HSPB1) cause axonal CMT or distal hereditary motor neuropathy (distal HMN). We developed and characterized transgenic mice expressing
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