H0163

Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal

clone HDAC4-144, purified from hybridoma cell culture

• Número MDL: MFCD05665442
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: HDAC4-144, monoclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~140 kDa
• reatividade de espécies: human, rat, mouse
• validação aprimorada: independent; Learn more about Antibody Enhanced Validation
• concentração: ~2 mg/mL
• técnica(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using total cell extracts of NIH3T3 fibroblasts cells
• Isotipo: IgG2a
• nº de adesão UniProt: P56524
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... HDAC4(9759) ; mouse ... Hdac4(208727) ; rat ... Hdac4(363287)

Descrição

Descrição geral

Monoclonal Anti-Histone Deacetylase 4 (HDAC4) (mouse IgG2a isotype) is derived from the HDAC4-144 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human HDAC4 with C-terminal added lysine, conjugated to KLH. HDAC4 belongs to the class II of Mammalian HDACs.

Aplicação

Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting[1]
• immunocytochemistry
• immunoprecipitation

Ações bioquímicas/fisiológicas

Histone deacetylase (HDAC) catalyzes the deacetylation of histones. HDAC4 is implicated in transcriptional repression. It dynamically migrates between nucleus and cytoplasm through its nuclear import and export signals. Interaction of HDAC4 with 14-3-3 and myocyte enhancer factor-2 (Mef2) proteins disturbs such shuttling and thus directs HDAC4 to the cytoplasm and the nucleus, respectively.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable