GE17-6001-11
Immobiline® DryStrip pH 3-10
Cytiva 17-6001-11, pack of 7 cm
About This Item
Produtos recomendados
forma
solid
Características
wetted part: no
embalagem
pack of 7 cm
fabricante/nome comercial
Cytiva 17-6001-11
cor
Colorless
solubilidade
cold water: soluble (Insoluble in cold and hot water)
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Categorias relacionadas
Descrição geral
Immobiline® DryStrip gels are available in fourteen overlapping pH ranges and five strip lengths (7, 11, 13, 18, and 24 cm) and represent the most comprehensive selection of narrow-, medium-, and wide-range IPG strips available. The five narrow pH range (1 pH unit), 24-cm IPG strips deliver the highest resolution and protein loading capacity available, which results in well separated spots with high protein content after the second-dimension separation.
The pH 3–11 NL strip covers most proteins found in prokaryotic and eukaryotic cells while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear to distribute the proteins evenly over the gel length to obtain maximal resolution.
Immobiline® DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of protein. Samples can also be loaded after rehydration. The first-dimension separation is carried out using Ettan™ IPGphor 3 IEF system or Multiphor II flatbed system.
Aplicação
Características e benefícios
- Maximize resolution, loading capacity, and reproducibility of first-dimension isoelectric focusing (IEF).
- Choose from a comprehensive range of overlapping Immobiline® DryStrip gels that cover narrow (1 pH unit), medium (3–5 pH units), and broad (8 pH units) pH ranges.
- Select the broad pH 3–11 NL gradient Immobiline® DryStrip gel for fast and efficient screening to gain a broad overview of total protein distribution.
- For more detailed studies or when using greater protein loads, choose from four new overlapping strips covering the pH 3 to 11 range.
- Higher resolution can be obtained using narrow-range (1 pH unit) Immobiline® DryStrip gels.
- Use matching IPG buffers for optimized running conditions.
- Use IPG strips directly after reswelling without further manipulation.
- Barcoded strips for convenient sample and batch tracking.
Armazenamento e estabilidade
Nota de análise
Informações legais
Código de classe de armazenamento
13 - Non Combustible Solids
Classe de risco de água (WGK)
WGK 2
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Protocolos
2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.
This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.
This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products
This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.
Conteúdo relacionado
This page shows how to apply equilibrated Immobiline DryStrip gels
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