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Documentos Principais

GE17-5068-01

Sigma-Aldrich

SOURCE 15RPC ST 4.6/100

pkg of 1 column

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About This Item

Código UNSPSC:
41115700
NACRES:
SB.52
Preço e disponibilidade não estão disponíveis no momento.

ligand

matrix surface

Materiais

polypropylene column

Características

autoclavable 20 min at 121°C

embalagem

pkg of 1 column

Parâmetros

flow rate
0.5 ml/min - 2.5 ml/min flow rate
580 psi

técnica(s)

HPLC: suitable

tamanho do leito

4.6 mm × 100 mm

volume do leito

1.7 mL

D.I. da coluna

4.6 mm

Matriz

polymeric
polystyrene/divinylbenzene

Grupo ativo da matriz

polystyrene-divinylbenzene phase

Tamanho médio da partícula

15 μm

cleaning

1-14

Faixa de trabalho

1-12

capacidade

~10 mg binding capacity

adequação

suitable for bioprocess medium

técnica de separação

reversed phase

Descrição geral

SOURCE 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.
~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h

Aplicação

SOURCE 15RPC is based on rigid, monosized 15 μm diameter polystyrene/divinyl benzene beads. The matrix has outstanding selectivity for RPC. The monodispersity of the beads yields stable beds, Low back pressures and excellent results at high flow rates.With its high physical and chemical stability and very high batch-to-batch reproducibility, SOURCE 15RPC is well suited for all stages of an industrial scale operation - from research and process development through scale-up and into production. SOURCE 15RPC offers properties superior to those of other polymeric matrices.

Características e benefícios

  • Wide pH range (1-12), outstanding selectivity, chemical resistance, high capacity, and high resolution at high fLow rates.
  • Excellent scalability, from RESOURCE to FineLINE columns.
  • using ÄKTA® design and other high-performance liquid chromatography systems.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Outras notas

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

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Informações legais

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


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Certificados de análise (COA)

Lot/Batch Number

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Artigos

“Ghosting” in chromatograms due to poor eluent quality; trace organic impurities concentrate, causing unknown peaks.

The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up

This page discusses column packing and preparation techniques for reverse phase chromatography.

Protocolos

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

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