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Documentos Principais

GE17-0405-01

HiTrap® Protein G High Performance

Cytiva 17-0405-01, pack of 1 × 5 mL

Sinônimo(s):

Protein G affinity column, Protein purification column

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

embalagem

pack of 1 × 5 mL

fabricante/nome comercial

Cytiva 17-0405-01

condição de armazenamento

(20% Ehtanol)
(20% Ethanol)

Parâmetros

flow rate
42 psi

tamanho do leito

16 mm × 25 mm

volume do leito

5 mL

D.I. da coluna

16 mm

Matriz

highly cross-linked 6% agarose

Tamanho médio da partícula

34 μm

cleaning

2-9

Faixa de trabalho

3-9

capacidade

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
125 mg binding capacity(human IgG/column)

temperatura de armazenamento

2-8°C

Descrição geral

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Características e benefícios

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


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Artigos

Designing purification methods for antibodies ensures quality and quantity adequacy for research and industrial purposes.

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

Ver tudo

Protocolos

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

General instructions for affinity purification using HiTrap columns from Cytiva.

This page shows how to prepare samples for purification with affinity chromatography.

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