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GE17-0402-03

HiTrap® Protein A High Performance

Cytiva 17-0402-03, pack of 2 × 1 mL

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About This Item

Código UNSPSC:
23151816
NACRES:
NA.56
Preço e disponibilidade não estão disponíveis no momento.

ligand

native protein A

embalagem

pack of 2 × 1 mL

fabricante/nome comercial

Cytiva 17-0402-03

condição de armazenamento

(20% Ethanol)

Parâmetros

42 psi

tamanho do leito

7 mm × 25 mm

volume do leito

1 mL

D.I. da coluna

7 mm

tamanho

Tamanho médio da partícula

34 μm

cleaning

2-10

Faixa de trabalho

3-9

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

HiTrap® protein A HP is a prepacked protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Aplicação

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.

Características e benefícios

  • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
  • 34 μm bead size for high-performance, high-resolution purifications.
  • Designed for use with syringe, pump, or chromatography system such as ÄKTA® design or FPLC™ System.
  • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
  • Robust, easy-to-use method.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

produto relacionado

Nº do produto
Descrição
Preços

Pictogramas

Flame
GHS02

Palavra indicadora

Warning

Frases de perigo

H226

Código de classe de armazenamento

3 - Flammable liquids

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Certificados de análise (COA)

Lot/Batch Number

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Artigos

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP

This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolos

General Instructions for Affinity Purification Using HiTrap® Columns

General instructions for affinity purification using HiTrap columns from Cytiva.

Protein A-Based Separation with Cytiva Products

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

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