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Key Documents

D5905

Sigma-Aldrich

3,3′-Diaminobenzidine tetrahydrochloride

peroxidase substrate, chromogenic, tablet

Sinônimo(s):

3,3′,4,4′-Biphenyltetramine tetrahydrochloride, 3,3′,4,4′-Tetraaminobiphenyl tetrahydrochloride, DAB

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About This Item

Fórmula linear:
(NH2)2C6H3C6H3(NH2)2 · 4HCl
Peso molecular:
360.11
Beilstein:
1212988
Número CE:
Número MDL:
Código UNSPSC:
12352204
ID de substância PubChem:
NACRES:
NA.83

product name

3,3′-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet

Nível de qualidade

forma

tablet

solubilidade

water: 1 tablet/15 mL (may contain small insoluble particles)

temperatura de armazenamento

−20°C

cadeia de caracteres SMILES

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

chave InChI

KJDSORYAHBAGPP-UHFFFAOYSA-N

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Especificidade

Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Aplicação

3,3′-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.
Reagent for spectrophotometric determination of selenium.

Ações bioquímicas/fisiológicas

3,3′-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.

Reconstituição

Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 μl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.

Pictogramas

Health hazardExclamation mark

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Código de classe de armazenamento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de risco de água (WGK)

WGK 3

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


Certificados de análise (COA)

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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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