CRISPR12V
CRISPR-Lenti Non-Targeting Control Transduction Particles
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About This Item
Código UNSPSC:
41106609
NACRES:
NE.02
Produtos recomendados
forma
liquid
embalagem
vial of 200 μL
concentração
1x106 VP/ml (via p24 assay)
aplicação(ões)
CRISPR
Condições de expedição
dry ice
temperatura de armazenamento
−70°C
Categorias relacionadas
Descrição geral
The Sigma lenti CRISPR Non-Targeting Control is a lentiviral system, which includes a gRNA sequence that does not target known human, mouse and rat genes. The non-targeting control lentivirus is useful as a negative control in experiments using Sigma CRISPR lentiviral clones. The Sigma lenti CRISPR non-targeting control uses a one-plasmid system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA (non-targeting), with both Puromycin and GFP co-expressed with Cas9.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001).
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001).
Aplicação
CRISPR-Lenti Non-Targeting Control Transduction Particles has been used in the transduction of human embryonic kidney 293 luciferase expressing cells with lentiviral particles, isolation of single colonies and MOSPD2 (motile sperm domain containing protein 2) silencing.
Functional Genomics/Screening/Target Validation
To see more application data, protocols, vector maps visit sigma.com/crisprs.
- Non-targeting control, CRISPR lentivirus, viral format, for creating gene knockouts/genetic modifications in cell lines.
To see more application data, protocols, vector maps visit sigma.com/crisprs.
Características e benefícios
Serves as a non-targeting or negative experimental control (lentiviral format) for the CRISPR editing workflow using lenti CRISPRs. This allows you to examine the effect of transduction in your system with the Sigma lenti CRISPR/Cas9 virus.
forma física
200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
Informações legais
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Protocolos
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
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