Human embryo kidney, infected with endogenous retrovirus of pigs
Descrição de linhagem celular
293-PERV-PK-CIRCE has been established by infecting the human kidney 293 cell line (Sigma Catalogue number. 85120602) with retrovirus produced by the pig kidney cell line PK-15. The endogenous, C-type retrovirus particles produced by PK-15 have been shown to be capable of infection, replication and serial transfer. 293-PERV-PK-CIRCE is useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation. The cell line has tested negative for Hog Cholera virus. Handle under ACDP Category 2 conditions.
Aplicação
Pig retrovirus studies, infectivity assays
Meio de cultura
DMEM + 2mM Glutamine + 10% HI-FBS
Rotina de subcultura
Split sub-confluent cultures (70-80%) 1:6 cells can be dislodged by mechanical agitation 5% CO2 3
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