NFS-25 C-3

93090713, mouse blood, Lymphoblast

• Código UNSPSC: 41106514

Propriedades

• product name: NFS-25 C-3, 93090713
• fonte biológica: mouse blood
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblast
• produtos: Not specified
• receptores: Ly-17, Lyb-2, Ly-1, Ly-5 (B220)
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse pre-B lymphoblast

Descrição de linhagem celular

Derived from a spontaneous lymphoma in an NFS.C58v-1 mouse. These pre-B cells are negative for surface Ig, Ia and ThB.

Meio de cultura

DMEM + 10% NCTC 109 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 1mM Oxaloacetic acid + 50μM 2-Mercaptoethanol (2ME) + 2μg/ml Transferrin + 0.2IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell

Outras notas

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