99072804

T-3-CI-2-0.fl cell line

mouse spleen, Lymphoblastoid-like

• Código UNSPSC: 41106514
• NACRES: NA.81

Propriedades

• product name: T-3-CI-2-0.fl cell line,
• fonte biológica: mouse spleen
• forma: liquid
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid-like
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse friend leukemia lymphoblast

Descrição de linhagem celular

T-3-Cl-2-0.fl is a clone of T-3-Cl-2, which originated from a splenic lesion of Friend virus-induced leukemia in a DD mouse. The cell line can be induced to differentiate into erythroblastoid cells with 1.5% DMSO. T-3-Cl-2-0.fl has been eradicated from mycoplasma using MC-210 by the originator.

Aplicação

Study of erythroid differentiation

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 3-9 x 100,000 cells/ml; 5%CO₂; 37ºC

Outras notas

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Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable